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and HRP enzyme conjugate are sequentially added to microwells pre-coated with a matrix metalloproteinase 19 (MMP19) capture antibody
suspended with 1 × Assay Buffer
DKFZp586H0523
1 mL of purified mitochondria with a total protein content of 10-100 μg
CDF Protein
His Tag ELISA Kit (Advanced) Molecular Biology Kits and HRP enzyme conjugate areProduct Specification Description This kit adopts competitive ELISA method, which can specifically detect HIS tag protein, and is used for quantitative detection of recombinant protein with HIS tag. Composition Item Quantity Storage Condition HRP conjugated anti His tag Antibody 10X 600 L 2 8C His tag protein Lyophilized Standards 2 vials 2 8C Assay Diluent #1 50 mL 2 8C Wash Buffer 10X 20 mL 2 8C TMB Solution 12 mL 2 8C Stop Solution 12 mL 2 8C Pre