DNA Pull Down Kit(Animal) Size:6T The activation of p44/42 MAPK

The activation of p44/42 MAPK involves a cascade of phosphorylation events

Expression System HEK293 Molecular Weight Theoretical: 21kDa Actual: 22-30kDa Purity ＞95% by SDS-PAGE

There was a positive correlation between the depth of color and the monkey Fcγ protein in the sample

Host Rabbit Synonyms Neurofibrillary tangle protein

Within these cells

DNA Pull Down Kit(Animal) Size:6T The activation of p44/42 MAPKProduct Specification Usage A, Total protein extraction 1. Cell samples (1) washing: precooling 1 ml of PBS washing sample (about 2 x 107 cells) 2 times, the last blot PBS as far as possible; (2) pyrolysis: according to the amount of cells to join 980 ul Lysis buffer, 10 ul Protease inhibitors (100 x) fully, the ice cracking 30 min, once every 5 min vortex, 10 s time; (3) Using ultrasonic cell breaker for 5min, power 20%, ultrasonic 3s, intermittent