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Tissue samples: Add lysate at a ratio of 100ul lysate per 3-10mg tissue
then add Assay Buffer and observe under fluorescence microscope (Calcein AM is green fluorescence
2、Noda et al
blow with pipetting gun to completely dissolve the RNA
⑤ Color development solution: According to 100uL per hole
Biotin Rat Anti-Mouse CD8α Antibody (S-353-45) Size:50T Tissue samples: Add lysate atProduct Specification Host Rat Antigen CD8 Synonyms T cell surface glycoprotein CD8 alpha chain; T cell surface glycoprotein Lyt 2; Lyt 2; Lyt2 Immunogen Recombinant Protein Location Cell membrane Accession P01731 Clone Number S 353 45 Antibody Type Rat mAb Isotype IgG1 Application FCM Reactivity Ms Positive Sample C57BL 6 mouse splenocytes Purification Protein G Concentration 0. 2 mg ml Conjugation Biotin Physical Appearance Liquid Storage Buffer PBS
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